Culture of previously uncultured members of the human gut microbiota by culturomics.

ABSTRACT: Metagenomics revolutionized the understanding of the relations among the human microbiome, health and diseases, but generated a countless number of sequences that have not been assigned to a known microorganism¹. The pure culture of prokaryotes, neglected in recent decades, remains essential to elucidating the role of these organisms². We recently introduced microbial culturomics, a culturing approach that uses multiple culture conditions and matrix-assisted laser desorption/ionization-time of flight and 16S rRNA for identification². Here, we have selected the best culture conditions to increase the number of studied samples and have applied new protocols (fresh-sample inoculation; detection of microcolonies and specific cultures of Proteobacteria and micro

SUBMITTER: Lagier JC 

PROVIDER: S-EPMC12094094 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature