When less is more: Counterintuitive stoichiometries and cellular abundances are essential for ABC transporters' function.

ABSTRACT: Prokaryotes acquire essential nutrients primarily through adenosine triphosphate-binding cassette (ABC) importers, consisting of an adenosine triphosphatase, a permease, and a substrate-binding protein. These importers are highly underrepresented in proteomic databases, limiting our knowledge about their cellular copy numbers, component stoichiometry, and the mechanistic implications of these parameters. We developed a tailored proteomic approach to compile the most comprehensive dataset to date of the Escherichia coli "ABC importome." Functional assays and analyses of deletion strains revealed mechanistic features linking molecular mechanisms to cellular abundances, colocalization, and component stoichiometries. We observed four to five orders of magnitude variation in import syste

SUBMITTER: Abdullah HQ 

PROVIDER: S-EPMC12094219 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature