Empowering the NSC-34 cell line as a motor neuron model: Cytosine arabinoside's action.

ABSTRACT: JOURNAL/nrgr/04.03/01300535-202601000-00039/figure1/v/2025-06-09T151831Z/r/image-tiff The NSC-34 cell line is a widely recognized motor neuron model and various neuronal differentiation protocols have been exploited. Under previously reported experimental conditions, only part of the cells resemble differentiated neurons; however, they do not exhibit extensive and time-prolonged neuritogenesis, and maintain their duplication capacity in culture. The aim of the present work was to facilitate long-term and more homogeneous neuronal differentiation in motor neuron-like NSC-34 cells. We found that the antimitotic drug cytosine arabinoside promoted robust and persistent neuronal differentiation in the entire cell population. Long and interconnecting neuronal processes with abundant growth cones

SUBMITTER: Vitale G 

PROVIDER: S-EPMC12094541 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature