HIF-1α modulates pancreatic cancer ECM proteins via the TGF-β1/Smad signaling pathway introduction.

ABSTRACT:

Introduction

Pancreatic cancer is characterized by its aggressive nature and poor prognosis, ranking among the most lethal malignancies. The tumor microenvironment, particularly the extracellular matrix (ECM), plays a crucial role in cancer progression. This study investigated the relationship between hypoxia-inducible factor-1α (HIF-1α) and transforming growth factor-β1 (TGF-β1) in regulating ECM protein expression in pancreatic cancer.

Methods

PANC-1 cells were cultured under both normoxic and hypoxic conditions. Pharmacological inhibition of HIF-1α and TGF-β1, as well as TGF-β1 stimulation, were employed to evaluate ECM protein expression. HIF-1α knockdown experiments and co-immunoprecipitation were performed to assess molecular interactions. Clinical specimens were analy

SUBMITTER: Guo H 

PROVIDER: S-EPMC12094911 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature