Develop an indirect ELISA utilizing gD protein to detect antibodies against bovine herpesvirus type 1.

ABSTRACT: Bovine herpesvirus type 1 (BHV-1) is a highly contagious DNA virus that causes a variety of diseases affecting the reproductive and respiratory tracts. These diseases can reduce the health and production performance of cattle, causing significant economic losses in the cattle industry. The current ELISA kits used to detect BHV-1 have long lead times and are expensive, and are not suitable for bulk testing on large farms. therefore, there is an urgent need to develop a rapid and cost-effective alternative to the BHV-1 test. In this study, recombinant gD protein was expressed by prokaryotic system, and then used as antigen to immunize New Zealand white rabbits to obtain polyclonal antibodies (pAb). An indirect enzyme-linked immunosorbent assay (iELISA) based on gD protein was established for

SUBMITTER: Liu Q 

PROVIDER: S-EPMC12094974 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature