LC-MS/MS-guided discovery of japonamides C and D, two new cyclohexadepsipeptides, from the <i>Nicotiana tabacum</i>-derived endophytic fungus <i>Aspergillus japonicus</i> TE-739D.

ABSTRACT: Endophytic fungi belonging to the Aspergillus genus have received substantial attention due to their notable secondary metabolic potential. In this study, chemical investigations using LC-MS/MS-based molecular networking on the endophytic fungus Aspergillus japonicus TE-739D led to the discovery of two new cyclohexadepsipeptides, namely japonamides C (1) and D (2), along with three known cyclodipeptides (3-5). Their structures, including the absolute configurations of the amino acid residues, were elucidated through spectroscopic data analysis and an optimized Marfey's method. The newly discovered compounds, japonamides C (1) and D (2), were screened for broad-spectrum cell proliferation inhibitory activity against 20 different human

SUBMITTER: Wang H 

PROVIDER: S-EPMC12095196 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature