HalALMT1 mediates malate efflux in the cortex of mature cluster rootlets of Hakea laurina, occurring naturally in severely phosphorus-impoverished soil.

ABSTRACT: Hakea laurina, a woody Proteaceae, naturally occurs in severely phosphorus (P)-impoverished habitats in southwest Australia. It develops distinctive cluster roots that exhibit a high capacity for carboxylate exudation and acid phosphatase activity, contributing to its P acquisition. However, the molecular mechanisms underlying these physiological functions remain poorly understood. We explored the cluster-root transcriptome using de novo RNA-Seq and identified Hakea laurina Aluminum-activated Malate Transporter 1 (HalALMT1), encoding an aluminum (Al)-activated malate transporter induced in mature cluster roots. We characterized HalALMT1 through electrophysiological assays and overexpression in Arabidopsis thaliana, and localized HalALMT1 expression, acid phosphatase activity, and suberized

SUBMITTER: Yamada H 

PROVIDER: S-EPMC12095975 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature