Arabidopsis GH3.10 conjugates jasmonates.

ABSTRACT: Jasmonates regulate plant development and defence. In angiosperms, the canonical bioactive jasmonate is jasmonoyl-isoleucine (JA-Ile), which is formed in Arabidopsis thaliana by JAR1 and GH3.10. In contrast to other jasmonate biosynthesis or perception mutants, however, gh3.10 jar1 knockout lines are still fertile. Therefore we investigated whether further jasmonates and GH3 enzymes contribute to regulation of fertility. Jasmonate levels were analysed by liquid chromatography-mass spectrometry. The substrate range of recombinant GH3.10 and related GH3 enzymes was studied using non-targeted ex vivo metabolomics with flower and leaf extracts of A. thaliana and in vitro enzyme assays. Jasmonate application experiments were performed to study their potential bioactivity. In flowers and wounded

SUBMITTER: Ni B 

PROVIDER: S-EPMC12096059 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature