Expanding the σ54-dependent transcription process with orthogonal designs.

ABSTRACT: The significance of orthogonal gene expression lies in its ability to ensure consistent and predictable operation of genetic pathways in synthetic biology. In bacteria, σ factors are responsible for promoter recognition, where the recognition pattern of σ54 is distinct from that of σ70. Moreover, σ54-dependent promoters require bacterial enhancer-binding proteins (bEBPs) for transcription initiation, which are stringently regulated and strongly activated. Thus, σ54 appears to be a promising candidate for orthogonal designs. In this study, through knowledge-based screening and rewiring of the RpoN box in σ54, together with its partnered promoter, we identified three sets of orthogonal expression systems based on σ54-R456H, R456Y, and R456L, with different promoter preferences and ideal mutu

SUBMITTER: Liu Y 

PROVIDER: S-EPMC12096077 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature