Effects of USP25 knockout on the gut microbial diversity and composition in mice.

ABSTRACT:

Background

The gut microbiota plays a crucial role in host health. Recent study revealed that ubiquitin-specific protease 25 (USP25) deficiency affected colonic immune responses and resistance to certain bacterial infection. This study aimed to investigate the impact of USP25 gene deletion on the gut microbiota of mice, utilizing 16 S rRNA amplicon sequencing and metagenomic sequencing to provide a comprehensive analysis of microbial diversity, composition and functional characteristics.

Methods

We collected fecal samples from 10 wild type (WT) C57BL/6J mice and 10 USP25^-/- mice (C57BL/6J-Usp25^em1cyagen) for 16 S rRNA amplicon sequencing. Subsequently, the 6 of the 20 samples underwent further analysis using metagenomic sequencing.

Results

Our re

SUBMITTER: Li J 

PROVIDER: S-EPMC12096529 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature