Prediction of lipase-specific foldase-dependence in bacterial lipase subfamilies I.1 and I.2.

ABSTRACT:

Background

Most bacterial lipases in subfamily I.1/I.2 depend on a specific chaperone protein, lipase-specific foldase (Lif), for folding into their active form. In contrast, several Lif-independent lipases have been reported in subfamily I.1. Lif-independent lipases have the potential to be industrially useful owing to their ease of heterologous expression; however, no method has been reported to predict Lif-dependence for an arbitrary lipase. In this study, we comprehensively estimated the Lif-dependence of subfamily I.1/I.2.

Results

To estimate Lif-dependence, we comprehensively analyzed the presence or absence of Lif genes in the genomes of bacteria from which the lipases were derived and integrated the results with those of phylogenetic analysis. We identified a range o

SUBMITTER: Hioki T 

PROVIDER: S-EPMC12096544 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature