SRSF1-mediated alternative splicing regulates bladder cancer progression and cisplatin sensitivity through HIF1A/BNIP3/mitophagy axis.

ABSTRACT:

Background

Alternative splicing (AS) is consistently linked to tumor progression. SRSF1, the first identified proto-oncogene in the serine/arginine-rich splicing factor (SRSF) protein family, plays a crucial role. However, the specific functions and potential mechanisms of SRSF1 in advancing bladder cancer (BCa) progression and influencing chemosensitivity remain largely unexplored.

Methods

The expression of SRSF1 in BCa tissues and cell lines was investigated using quantitative real-time PCR (RT-qPCR) and western blotting. Survival analysis was employed to examine the association between SRSF1 expression and prognosis of BCa. The functions of SRSF1 were evaluated through proliferation assays, migration assays, IC50 determination assays, and tumorigenesis assays in nude mice

SUBMITTER: Wu Q 

PROVIDER: S-EPMC12096585 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature