Functional insights from recombinant production of bacterial proteases in Saccharomyces cerevisiae.

ABSTRACT:

Background

Proteases are important enzymes in food and pharmaceutical industries, but challenges persist in their recombinant production due to host cell proteome hydrolysis and fitness loss. The development of recombinant expression systems for directed evolution of proteolytic enzymes, and industrial production are desirable. This study evaluated Saccharomyces cerevisiae as expression host for three bacterial proteases: BdpK (from Bdellovibrio bacteriovorus), IdeS, and SpeB (both from Streptococcus pyogenes), each with distinct peptide substrate scopes.

Results

We developed an experimental pipeline for analysis of protease gene expression levels and fitness effects on yeast cultures. Heterologous genes were fused with green fluorescent protein and their expression and effe

SUBMITTER: Lindh T 

PROVIDER: S-EPMC12096733 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature