The adjacent ATP-binding protein-encoding genes of the <i>Enterococcus faecalis</i> phosphate-specific transport (<i>pst</i>) locus have non-overlapping cellular functions.

ABSTRACT: The widely conserved pst-phoU operon encodes a low-velocity, high-affinity, ATP-dependent importer for inorganic phosphate (Pi). The pstB gene encodes the ATPase that powers the import of Pi into the cell. In some Firmicutes, including the gastrointestinal commensal and opportunistic pathogen Enterococcus faecalis, the pst-phoU locus contains adjacent pstB genes. In this work, we compared the functionality of E. faecalis pstB1 and pstB2. E. faecalis pstB1 and pstB2 share sequence similarities with verified PstB ATPases from Escherichia coli and Streptococcus pneumoniae and only share ~60% amino acid identity with each other. Deletion of pstB1 was associated with a growth defect in low Pi-containing chemically defi

SUBMITTER: Healy CM 

PROVIDER: S-EPMC12096839 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature