The Dynamic Landscape of 3'-UTR Alternative Polyadenylation Across Mouse Fetal Development and Anatomy.

ABSTRACT: Alternative cleavage and polyadenylation (APA) in the 3'-untranslated region (3'-UTR) of mRNA produces transcripts with varied 3'-UTR and plays a key role in development and organogenesis. This work characterizes 3'-UTR APA using 85 high-quality RNA-seq datasets encompassing 12 tissue types and eight developmental stages of mouse fetuses. Results show that 46.4% of expressed genes undergo APA in a tissue-specific manner. Changes in polyadenylation site (pAS) usage often operate beyond transcriptional control, revealing APA as an additional layer of gene regulation. Sequence analysis demonstrates that pAS selection, governed by polyadenylation signal strength and adenine preferences, is evolutionarily conserved between mice and humans. Intriguingly, brain tissues display complex 3'-UTR APA

SUBMITTER: Wang Q 

PROVIDER: S-EPMC12097126 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature