Chronic optogenetic stimulation of dentate gyrus granule cells in mouse organotypic slice cultures synaptically drives mossy cell degeneration.

ABSTRACT:

Objective

Degeneration of hilar mossy cells in the dentate gyrus is an important hallmark of hippocampal sclerosis and is often observed in patients with temporal lobe epilepsy. To understand the pathogenesis of hippocampal sclerosis and develop novel neuroprotective treatments, it is critical to determine the mechanistic processes of mossy cell degeneration and factors that influence cell vulnerability or resilience. However, suitable in vitro approaches are currently lacking. We have developed and validated an organotypic slice culture-based in vitro model that facilitates mechanistic studies of activity-dependent mossy cell vulnerability and resilience.

Methods

A model was developed using entorhino-hippocampal slice cultures. Dentate gyrus granule cells were transduced wi

SUBMITTER: Koretz CC 

PROVIDER: S-EPMC12097467 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature