Long-term multi-meta-omics resolves the ecophysiological controls of seasonal N₂O emissions during wastewater treatment.

ABSTRACT: Nitrous oxide (N₂O) is the third most important greenhouse gas and originates primarily from natural and engineered microbiomes. Effective emission mitigations are currently hindered by the largely unresolved ecophysiological controls of coexisting N₂O-converting metabolisms in complex communities. To address this, we used biological wastewater treatment as a model ecosystem and combined long-term metagenome-resolved metaproteomics with ex situ kinetic and full-scale operational characterization over nearly 2 years. By leveraging the evidence independently obtained at multiple ecophysiological levels, from individual genetic potential to actual metabolism and emergent community phenotype, the cascade of environmental and operational triggers driving seasonal N_2</

SUBMITTER: Roothans N 

PROVIDER: S-EPMC12098122 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature