<i>Arabidopsis thaliana</i> Roots Exposed to Extracellular Self-DNA: Evidence of Epigenetic Effects.

ABSTRACT:

Background

Previous evidence demonstrated DNA methylation changes in response to stress in plants, showing rapid changes within a limited time frame. Exposure to self-DNA inhibits seedling root elongation, and it was shown that it causes changes in CG DNA methylation in Lactuca sativa. We assessed cytosine methylation changes and associated gene expression patterns in roots of Arabidopsis thaliana Col-0 seedlings exposed to self-DNA for 6 and 24 h.

Methods

We used whole genome bisulfite sequencing (WGBS) and RNA-seq analyses to assess genomic cytosine methylation and corresponding gene expression, respectively, on DNA and RNA extracted with commercial kits from roots exposed to self-DNA by an original setup. Fifteen hundred roots replicates, including the contr

SUBMITTER: Ronchi A 

PROVIDER: S-EPMC12101399 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature