MultiPassMerger: Automated data processing for multipass cyclic ion mobility HDX-MS.

ABSTRACT: Hydrogen/deuterium exchange-mass spectrometry (HDX-MS) is a powerful tool for studying protein structure and dynamics. As a bottom-up LC-MS technique, its success largely depends on peptide identifications made by peptide mapping prior to HDX measurements. We previously demonstrated that combining peptide mapping results from complementary single- and multipass cyclic ion mobility-mass spectrometry (cIM-MS) experiments, an approach we term "multi-sequence" cIM-MS, can enhance HDX-MS by increasing peptide identifications. However, this approach required labor-intensive, manual handling of the acquired data, including lengthy optimization of drift time (DT) versus DT full width half maximum (FWHM) trendlines during peak detection processing to combat cyclic wrap-around effects. Here, we pres

SUBMITTER: Duerr V 

PROVIDER: S-EPMC12102732 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature