Promoter engineering for enhanced 3-hydroxypropionic acid production in <i>Pichia pastoris</i>.

ABSTRACT: Enabling tools are essential for facilitating the methanol bioconversion in Pichia pastoris. However, there is still a relative lack of promoters that can stably express high levels without being affected by the carbon source, which hinders the construction and modification of cell factories containing long metabolic pathways. This study mapped a gene expression intensity library of central metabolic pathways in P. pastoris under methanol and glucose conditions. Through modification of the upstream sequences of promoters, an artificial promoter P _S2 was developed with a strong intensity up to 90 % of P _GAP . By using this promoter, we successfully constructed a hybrid pathway that integrates the β-alanine and malonyl-CoA pathways for the pr

SUBMITTER: Chen S 

PROVIDER: S-EPMC12104163 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature