Development of cleaved amplified polymorphic sequence marker for powdery mildew resistance in Korean malting barley using QTL-seq.

ABSTRACT:

Introduction

Powdery mildew (PM) caused by Blumeria graminis f.sp. hordei is a major fungal disease affecting barley (Hordeum vulgare L.). The most effective approach to controlling this disease is the development of resistant cultivars. In this study, we investigated the genomic regions associated with PM resistance by performing quantitative loci sequencing (QTL-seq) twice using the parental lines 'Hopum' (susceptible) and 'Jeonju 182' (resistant) as reference genomes.

Methods

This study was conducted from 2022 to 2024 at the National Institute of Agricultural Sciences in Wanju, Republic of Korea. We conducted artificial crossing, genomic DNA extraction, phenotypic evaluation, QTL-seq analysis, and cleaved amplified polymorphic sequence (CAPS) marker d

SUBMITTER: Park JC 

PROVIDER: S-EPMC12104202 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature