Deciphering the Genic Basis of Yeast Fitness Variation by Simultaneous Forward and Reverse Genetics.

ABSTRACT: The budding yeast Saccharomyces cerevisiae is the best studied eukaryote in molecular and cell biology, but its utility for understanding the genetic basis of phenotypic variation in natural populations is limited by inefficient association mapping due to strong and complex population structure. To overcome this challenge, we generated genome sequences for 85 strains and performed a comprehensive population genomic survey of a total of 190 diverse strains. We identified considerable variation in population structure among chromosomes and identified 181 genes that are absent from the reference genome. Many of these nonreference genes are expressed and we functionally confirmed that two of these genes confer increased resistance to antifungals. Next, we simultaneously measured the growth rat

SUBMITTER: Maclean CJ 

PROVIDER: S-EPMC12104513 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature