Impact of NA-1 on Pericyte-Driven Vasoconstriction and Its Role in No-Reflow During Cerebral Ischemia-Reperfusion.

ABSTRACT:

Background

The no-reflow phenomenon in the ischemic brain following arterial recanalization leads to poor prognosis. Previous studies suggest that the clinically proven NA-1 drug, with disruption of PSD95 in the neuronal terminals alongside the cerebral microvasculature, may inhibit pericytic contraction of microvessels by reducing endothelin-1 secretion.

Methods

A 1.5-h tMCAO model using Balb/c mice was employed. In vivo two-photon imaging and immunofluorescence staining were employed to assess the constriction effect of pericytes on capillaries. The impact of NR2B9c/NA-1 administration (intravenously infused at a dose of 10 μmol/kg) on pericyte constriction was evaluated through immunofluorescent staining of brain sections. Additionally, The effect of NA-1 on cerebral perf

SUBMITTER: Yang X 

PROVIDER: S-EPMC12104562 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature