Allantofuranone Biosynthesis and Precursor-Directed Mutasynthesis of Hydroxylated Analogues.

ABSTRACT: Genome mining and heterologous reconstitution of biosynthetic genes in Aspergillus oryzae enabled elucidation of the hitherto elusive biosynthetic route that produces allantofuranone (1), a bioactive natural product originally isolated from Allantophomopsis lycopodina. The core non-ribosomal peptide synthetase (NRPS)-like enzyme AlfA of the alf BGC produces polyporic acid (2) from phenylpyruvic acid. In subsequent reactions, compound 2 is reductively dehydrated by the bifunctional enzyme AlfC and methylated by AlfD to produce terferol (6). In a final step, the quinol moiety of compound 6 is oxidatively cleaved and contracted by the aromatic ring cleavage dioxygenase AlfB. Using combinatorial biosynthesis, we were able to manipulate th

SUBMITTER: Wieder C 

PROVIDER: S-EPMC12105029 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature