Colorimetric Optode Sensor with Tripodal Ionophore for Rapid Urinary Ammonium Determination.

ABSTRACT: The amount of ammonium excreted in urine is a crucial indicator for assessing renal metabolic acidosis. Therefore, there is a significant demand for chemical sensors capable of accurately determining urinary ammonium concentrations with high throughput. In this study, we developed an ammonia chemical sensor based on the ion-selective optode principle. This sensor employs strategically designed ionophores and a PTFE gas-permeable film to enhance selectivity for free NH₃. The low basicity of the chromoionophore indicator and the weak affinity of the tripodal ammonium receptor (L, log β = 4.62 ± 0.18) contributed to a markedly reduced response time, achieving detection within seconds, a significant improvement over previous sensors. A limit of detection (LOD) of approximately 15 nM

SUBMITTER: Fu F 

PROVIDER: S-EPMC12105079 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature