Detection of Inverse Stable Isotopic Labeling in Untargeted Metabolomic Data.

ABSTRACT: Stable isotopic labeling is a powerful tool for determining the biosynthetic origin of metabolites and for discovering natural products that incorporate precursors of interest. When isotopically substituted precursors are not available commercially or synthetically, inverse stable isotopic labeling (InverSIL) is a useful alternative. With InverSIL, an organism is grown on an isotopically substituted medium and then fed precursors of natural isotopic abundance which can be tracked by mass spectrometry, thereby bypassing issues with precursor availability. Currently, there is no automated way to identify precursor incorporation in untargeted metabolomic data using InverSIL without specifying an expected change in the mass-to-charge ratio of metabolites that have incorporated the precursor. T

SUBMITTER: Liebergesell TCE 

PROVIDER: S-EPMC12105114 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature