Generation of Conformation-Specific Monoclonal Antibodies for Integral Membrane Proteins.

ABSTRACT: Antibodies and their antigen-binding fragments, including fragment antigen-binding domains (Fabs) and single-chain variable fragments (scFvs), are extraordinary tools in all fields of biology, particularly in neuroscience, where they have been utilized for imaging, detection, and quantification studies. Most antibodies bind to unstructured or linear epitopes. Conformation-specific antibodies, by contrast, bind to 3D epitopes, recognizing native conformations of the target antigen, and have proven highly useful in X-ray crystallography as crystallization chaperones and in cryo-electron microscopy as fiducial markers. Moreover, because conformation-specific antibodies recognize 3D shapes of the antigen, they often have exquisite specificity and are useful in immunofluorescence studies and in

SUBMITTER: Sheldon N 

PROVIDER: S-EPMC12105683 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature