Incomplete elimination of viral genomes is associated with chronic inflammation in nonhuman primate livers after AAV-mediated gene transfer.

ABSTRACT: The liver is a unique organ where immunity can be biased toward ineffective response notably in the context of viral infections. Chronic viral hepatitis depends on the inability of the T-cell immune response to eradicate antigen. In the case of recombinant Adeno-Associated-Virus, used for therapeutic gene transfer, conflicting reports describe tolerance induction to different transgene products while other studies have shown conventional cytotoxic CD8⁺ T cell responses with a rapid loss of transgene expression. We performed a 1 year follow up of 6 non-human primates after all animals received an rAAV8 vector carrying the GFP transgene at doses of 7×10¹² vg/kg. We report that despite anti-GFP peripheral cellular response and loss of hepatic transgene expression, we wer

SUBMITTER: Pichard V 

PROVIDER: S-EPMC12106068 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature