Screening and identification of protein interacting with goose astrovirus.

ABSTRACT:

Introduction

Goose Astrovirus (GoAstV), a recently identified member of the Astroviridae family in China, predominantly affects goslings, resulting in substantial economic losses to the goose farming industry due to its high infection and mortality rates. Currently, the infection mechanism and pathogenesis of GoAstV remain unknown.

Methods

Given this, the Viral Overlay Protein Blot Assay was utilized to identify and characterize proteins on the LMH (Leghorn Male Hepatoma) cell membrane that interact with Goose Astrovirus. The identities of the candidate proteins were determined via LC-MS mass spectrometry analysis, bioinformatics analysis, and UniProt database search. The interaction between HSPA5 and the astrovirus protein was further validated in vitro through Western blot

SUBMITTER: Qian L 

PROVIDER: S-EPMC12106297 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature