Genome-wide identification of the cotton ANK gene family and functional study of GhANK169 in response to heat stress.

ABSTRACT:

Background

High temperatures significantly impair the yield and quality of cotton. Ankyrin-repeat proteins (ANKs) are among the largest superfamilies of proteins in plants and play vital roles in both biotic and abiotic stress responses.

Results

In this study, we identified and characterized members of the cotton ankyrin repeat protein (ANK) gene family, focusing on the ANK-TM subfamily member GhANK169, which was highly upregulated in the RNA-seq data of cotton plants exposed to 42 °C. Overexpression of GhANK169 in tobacco at 42 °C resulted in improved phenotypic resilience, enhanced antioxidant enzyme activity, and reduced relative electrolyte leakage (REL) and malondialdehyde (MDA) content. Conversely, silencing GhANK169 in cotton via virus-induced gene silencing (VIGS) re

SUBMITTER: Hou M 

PROVIDER: S-EPMC12107779 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature