Virulence of fowl adenovirus (FAdV) serotype 4 strains impacts cell proliferation and immune response of primary chicken-embryo intestinal epithelial cells.

ABSTRACT: Fowl adenovirus serotype 4 (FAdV-4) causes hepatitis-hydropericardium syndrome (HHS) in chickens, leading to substantial economic losses. Following oral uptake, the virus infects intestinal epithelial cells (IEC) to overcome the first entrance barrier. The initial cellular interactions and intestinal immune responses are not well understood. This study uses a primary IEC culture model to investigate infection dynamics of virulent (AG234) and non-pathogenic (KR5) FAdV-4 strains and cellular defence mechanisms. Cell growth and viral propagation were assessed at 4, 12, 24, and 48 hours post-infection (hpi) using immunofluorescence and automated image analysis. The innate immune response was assessed by the mRNA expression of the Toll-like receptors (TLR1B, TLR2B, TLR3, TLR4, and TLR21) and th

SUBMITTER: Kau-Strebinger K 

PROVIDER: S-EPMC12107922 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature