A Lipidomic Approach to Studying the Downregulation of Free Fatty Acids by Cytosolic Phospholipase A₂ Inhibitors.

ABSTRACT: Inhibitors of cytosolic phospholipase A₂ (GIVA cPLA₂) have received great attention, since this enzyme is involved in a number of inflammatory diseases, including cancer and auto-immune and neurodegenerative diseases. Traditionally, the effects of GIVA cPLA₂ inhibitors in cells have been studied by determining the inhibition of arachidonic acid release. However, although to a lesser extent, GIVA cPLA₂ may also hydrolyze glycerophospholipids, releasing other free fatty acids (FFAs), such as linoleic acid or oleic acid. In the present work, we applied a liquid chromatography-high-resolution mass spectrometry method to study the levels of intracellular FFAs, after treating cells with selected GIVA cPLA₂ inhibitors. Six inhibitors belongin

SUBMITTER: Bourboula A 

PROVIDER: S-EPMC12108850 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature