The Wheat Intrinsically Disordered Protein <i>Td</i>RL1 Negatively Regulates the Type One Protein Phosphatase <i>Td</i>PP1.

ABSTRACT: Type 1 protein phosphatases (PP1s) are crucial in various plant cellular processes. Their function is controlled by regulators known as PP1-interacting proteins (PIPs), often intrinsically disordered, such as Inhibitor 2 (I2), conserved across kingdoms. The durum wheat TdRL1 acts as a positive regulator of plant stress tolerance, presumably by inhibiting PP1 activity. In this work, co-immunoprecipitation and bimolecular fluorescence complementation (BiFC) assays demonstrate that the durum wheat TdPP1 interacts with both TdRL1 and At-I2 in vivo. YFP fluorescence restored after TdRL1-TdPP1 interaction decorated specifically the microtubular network of the tobacco co-infiltrated cells. In vitro phosphatase assays revealed that TdRL1 inhibited t

SUBMITTER: Amor F 

PROVIDER: S-EPMC12109034 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature