PAX3 Regulatory Signatures and Gene Targets in Melanoma Cells.

ABSTRACT:

Background/objectives

PAX3 is a transcription factor that drives melanoma progression by promoting cell growth, migration, and survival, while inhibiting cellular terminal differentiation. However, known PAX3 target genes are limited and cannot fully explain the wide impact of PAX3 function. The PAX3 protein can regulate DNA through two separate binding domains, the Paired Domain (PD) and Homeodomain (HD), which bind different DNA motifs. It is not clear if these two domains bind and work together to regulate genes and if they promote all or only a subset of downstream cellular events.

Methods

PAX3 direct downstream targets were identified using Cleavage Under Targets & Release Using Nuclease (CUT&RUN) assays in SK-MEL-5 melanoma cells. PAX3-binding genomic regions were iden

SUBMITTER: Moore SPG 

PROVIDER: S-EPMC12111051 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature