Isolation and Activity Evaluation of Callus-Specific Promoters in Rice (<i>Oryza sativa</i> L.).

ABSTRACT: Background/Objectives: In crop genetic engineering, morphogenic genes have attracted increasing attention, given their ability to facilitate the transformation of a broad range of otherwise nontransformable cultivars. However, few callus-specific promoters have been identified to date that can be employed to avoid the adverse effects resulting from the ectopic expression of morphogenic genes on shoot regeneration and growth. Methods: A set of potential callus-specific genes were initially selected based on publicly available data. These genes were then screened using quantitative real-time polymerase chain reaction (qPCR), followed by promoter activity evaluation using a transgenic approach with the GUS gene serving as a reporter. Results: Of the 24 evaluated pr

SUBMITTER: Ma X 

PROVIDER: S-EPMC12111461 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature