Nanopore-based targeted next-generation sequencing (tNGS): A versatile technology specialized in detecting low bacterial load clinical specimens.

ABSTRACT:

Introduction

The 2024 global tuberculosis report indicated that the epidemiological situation of tuberculosis remains concerning. Current tuberculosis detection methods have limitations, highlighting the urgent need to develop more convenient, effective, and widely utilized detection technologies in clinical settings to facilitate early diagnosis and treatment guidance for tuberculosis. Nanopore-based targeted next-generation sequencing (tNGS) offers advantages such as convenience, efficiency, and long-read sequencing, making it a commonly used method for mycobacteria identification.

Methods

This study compared the detection efficiency of tNGS with Xpert MTB/RIF, MTB culture, and AFB smear in sputum samples, BALF samples, and pathological tissue samples to evaluate the clini

SUBMITTER: Yang C 

PROVIDER: S-EPMC12111578 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature