Capillary Electrophoresis Optimization for Metabolite Separation in <i>Hypogymnia physodes</i> Using DoE: Validation Across Lichen Species.

ABSTRACT: Lichen-specific natural products exhibit a wide range of biological activities, which makes them potentially useful in the pharmaceutical, cosmetic, and nutritional industries. In the present study, a capillary electrophoresis method was developed and optimized for the separation of seven major metabolites, physodic acid, 3-hydroxyphysodic acid, atranorin, physodalic acid, chloroatranorin, salazinic acid, and protocetraric acid, found in Hypogymnia physodes. The optimization was performed using a design of experiments approach, focusing on four critical parameters: boric acid concentration, deoxycholic acid concentration, methanol content, and buffer pH. The overall separation efficiency was used as the response factor for optimization. The optimal separation conditions were achieve

SUBMITTER: Dresler S 

PROVIDER: S-EPMC12112299 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature