Structural Characterisation of TetR/AcrR Regulators in <i>Streptomyces fildesensis</i> So13.3: An In Silico CRISPR-Based Strategy to Influence the Suppression of Actinomycin D Production.

ABSTRACT: The growing threat of antimicrobial resistance has intensified the search for new bioactive compounds, particularly in extreme environments such as Antarctica. Streptomyces fildesensis So13.3, isolated from Antarctic soil, harbours a biosynthetic gene cluster (BGC) associated with actinomycin D production, an antibiotic with biomedical relevance. This study investigates the regulatory role of TetR/AcrR transcription factors encoded within this biosynthetic gene cluster (BGC), focusing on their structural features and expression under different nutritional conditions. Additionally, we propose that repressing an active pathway could lead to the activation of silent biosynthetic routes, and our in-silico analysis provides a foundation for selecting key mutations and experimentally vali

SUBMITTER: Leal K 

PROVIDER: S-EPMC12112392 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature