MicroRNA Targeting Cytochrome P450 Is Involved in Chlorfenapyr Tolerance in the Silkworm, <i>Bombyx mori</i> (Lepidoptera: Bombycidae).

ABSTRACT: We first measured the content of chlorfenapyr and tralopyril in silkworm larvae using HPLC, revealing that chlorfenapyr can be biotransformed into tralopyril in silkworms. Then, a differential transcriptomic database of small RNA was constructed through Illumina RNA-Sequencing. qRT-PCR was conducted to determine the expression levels of Bmo-miR-6497-5p and the target CYP450 gene, and Bmo-miR-6497-5p was significantly upregulated in the L3 silkworm larvae 24, 48, and 72 h after they were treated with chlorfenapyr. Furthermore, the target P450 gene CYP337A2 was downregulated at these time points. Dual-luciferase validation revealed that the luciferase activity significantly decreased after Bmo-miR-6497-5p bound to CYP337A2. In addition, miRNA mimics/inhibitor injection and bioa

SUBMITTER: Shao Y 

PROVIDER: S-EPMC12112709 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature