Susceptibility of <i>Lactobacillaceae</i> Strains to Aminoglycoside Antibiotics in the Light of EFSA Guidelines.

ABSTRACT: Lactobacillaceae is a large family of bacteria from which probiotic strains often originate. Microorganisms used as feed additives in the EU must meet a number of formal criteria, some of which concern antimicrobial susceptibility. In this study, we determined the susceptibility of 19 reference strains and 121 wild-type strains of Lactobacillaceae to aminoglycoside antibiotics using the broth microdilution method based on the ISO 10932:2010/IDF 223:2010 standard. Strains were categorized as resistant or susceptible according to European Food Safety Authority (EFSA) guidelines. Resistance genes were detected by whole genome sequence (WGS) analysis or by PCR. The MICs read after 48 h of incubation showed that 36.8% of reference strains were resistant to kanamycin, 26.3% to stre

SUBMITTER: Dec M 

PROVIDER: S-EPMC12112790 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature