Eliciting Clavulanic Acid Biosynthesis: The Impact of <i>Bacillus velezensis</i> FZB42 on the Metabolism of <i>Streptoyces clavuligerus</i> ATCC 27064.

ABSTRACT: Background/Objectives: Clavulanic acid (CA) is produced by cell suspension cultures of Streptomyces clavuligerus ATCC 27064, and is widely used as a beta-lactamase inhibitor to combat antibiotic resistance. CA titers are moderate due to bioprocess complexity, prompting ongoing efforts to overcome these limitations. Methods: In this study, we aimed to evaluate the effect of live and inactivated Bacillus velezensis FZB42 cells on CA production in S. clavuligerus, and to explore the transcriptional response underlying this interaction using RNA-seq technology. Results: The addition of dead and live cells of B. velezensis improved CA production by 1.4 and 2.0-fold, respectively. Furthermore, the transcriptome of S. clavuligerus, obtained

SUBMITTER: Patino LF 

PROVIDER: S-EPMC12113186 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature