Phase transition of WTAP regulates m⁶A modification of interferon-stimulated genes.

ABSTRACT: N⁶-methyladenosine (m⁶A) is the most prevalent modification of mRNA which controls diverse physiological processes. Although m⁶A modification has been reported to regulate type I interferon (IFN) responses by targeting the mRNA of IFN-β and the interferon-stimulated genes (ISGs), the detailed mechanism of how m⁶A methyltransferase complex (MTC) rapidly responds to conduct the modification on nascent mRNA during IFN-β stimulation remains largely unclear. Here, we demonstrate that WTAP, the adaptor protein of m⁶A MTC, undergoes dephosphorylation-regulated phase transition from aggregates to liquid-like condensates under IFN-β stimulation, thereby mediating m⁶A modification of a subset of ISGs to restrict their expressio

SUBMITTER: Cai S 

PROVIDER: S-EPMC12113268 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature