Characterization of the VOC Promoter That Is Active Under Low-Salinity Conditions in the Diatom <i>Phaeodactylum tricornutum</i>.

ABSTRACT: Microalgae such as Phaeodactylum tricornutum are promising cell biofactories for the production of high-value molecules, including monoclonal antibodies (mAbs). However, to date, the production of mAbs in P. tricornutum using the inducible nitrate reductase (NR) promoter has yielded only a limited amount of mAbs. Therefore, the identification of a robust promoter that produces high yields of mAbs is crucial for the development of a cost-effective expression system. To date, only a few endogenous promoters have been characterized in P. tricornutum. In this study, we identified thirty-three potential "strong" endogenous promoters based on our previously published transcriptomic data from the P. tricornutum Pt3 strain. These putative promoter sequences were cloned

SUBMITTER: Toustou C 

PROVIDER: S-EPMC12113381 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature