Ureolysis-Driven Microbially Induced Carbonate Precipitation by a Facultatively Anaerobic Thermophilic Bacterium Under High-Temperature and Anaerobic Conditions.

ABSTRACT: Microbially induced carbonate precipitation (MICP) is the precipitation of CaCO₃ crystals, induced by microbial metabolic activities such as ureolysis. Various applications of MICP have been proposed as innovative biocementation techniques. This study aimed to verify the feasibility of ureolysis-driven MICP applications in deep-subsurface environments (e.g., enhanced oil recovery and geological carbon sequestration). To this end, we screened sludge collected from a high-temperature anaerobic digester for facultatively anaerobic thermophilic bacteria possessing ureolytic activity. Then, we examined the ureolysis-driven MICP using a representative isolate, Bacillus haynesii strain SK1, under aerobic, anoxic, and strict anaerobic conditions at 30 °C, 40 °C, and 50 °C. All cu

SUBMITTER: Shen X 

PROVIDER: S-EPMC12113697 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature