Efficient Production Strategy of a Novel Postbiotic Produced by <i>Bacillus subtilis</i> and Its Antioxidant and Anti-Inflammatory Effects.

ABSTRACT: Microbially synthesized postbiotics have unique properties and advantages; however, systematic studies on the efficient production and biological functions of postbiotics from Bacillus subtilis are limited, which greatly restricts their applications. In this study, we obtained a novel crude exopolysaccharide (EPS) postbiotic from Bacillus subtilis H4. We systematically optimized the EPS production strategy using single-factor analysis, Plackett-Burman design, the path of steepest ascent method, and response surface methodology. The optimized EPS yield was significantly improved, with a maximum yield of 15.01 g/L under the addition of 4.12% soy peptone, 8.99% sucrose, and 0.06% MnSO₄. We found that EPS is a neutral, heterogeneous polysaccharide with a pyranose ring,

SUBMITTER: Zhang J 

PROVIDER: S-EPMC12113817 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature