In Silico Design, Optimization, and Evaluation of a Multi-Epitope Vaccine Targeting the <i>Clostridium perfringens</i> Collagen Adhesin Protein.

ABSTRACT: Necrotic enteritis (NE), caused by pathogenic Clostridium perfringens, poses a significant threat to global poultry health, with estimated annual losses exceeding USD 6 billion. The rising incidence of NE has been associated with the reduced use of antibiotic growth promoters, underscoring the urgent need for alternative control measures such as vaccination. Collagen adhesin protein (CNA), a key virulence factor in NE pathogenesis, represents a promising vaccine target. The US Food and Drug Administration has begun phasing out animal testing requirements for biologics and monoclonal antibody drugs. In this study, a computational multi-epitope vaccine (MEV) targeting CNA was designed by integrating predicted Cluster of Differentiation (CD)4⁺ helper T lymphocyte (Th), CD8

SUBMITTER: Chundru D 

PROVIDER: S-EPMC12113974 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature