Periplasmic Protein Mobility for Extracellular Electron Transport in <i>Shewanella oneidensis</i>.

ABSTRACT: Extracellular electron transport (EET) supports the survival of specific microorganisms on the Earth's surface by facilitating microbial respiration with diverse electron acceptors. A key aspect of EET is the organization of electron relays, i.e., multi-heme c-type cytochromes (MHCs), within the periplasmic space of microbial cells. In this study, we investigated the mobility of periplasmic electron relays in Shewanella oneidensis MR-1, a model strain capable of EET, using in vivo protein crosslinking to the MHCs. First, we established that crosslinking efficiency correlates with the spatial proximity and diffusion coefficient of protein molecules through in vitro tests. Based on these findings, we identified distinct molecular behaviors of periplasmic MHCs, showing that the tetrahe

SUBMITTER: Li D 

PROVIDER: S-EPMC12114092 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature