Genome Mining Reveals Rifamycin Biosynthesis in a Taklamakan Desert Actinomycete.

ABSTRACT: Actinomycetes are recognized for producing diverse bioactive natural products, yet most biosynthetic gene clusters (BGCs) remain inactive under laboratory conditions. Rare actinomycetes from extreme environments represent underexplored reservoirs of metabolic potential. This study investigates Actinomadura sp. TRM71106, a rare actinomycete isolated from the Taklamakan Desert, through integrated genomic and metabolomic approaches. Genome sequencing revealed 45 secondary metabolic BGCs, including BGC38 showing 65% nucleotide similarity to the rifamycin BGC. Gene cluster networking and linear comparisons predicted its capacity to encode novel rifamycin analogs. Targeted activation strategies-overexpression of the pathway-specific regulator LuxR combined with metabolite isolation

SUBMITTER: Luo X 

PROVIDER: S-EPMC12114206 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature