LcTprxII Overexpression Enhances Physiological and Biochemical Effects in Maize Under Alkaline (Na₂CO₃) Stress.

ABSTRACT: Alkaline stress limits crop productivity by causing osmotic and oxidative damage. This study investigated the new gene LcTprxII, a type II peroxiredoxin encoded by Leymus chinensis, and its role in enhancing alkaline stress tolerance in transgenic maize. The gene was cloned, overexpressed, and characterized using RT-PCR, phylogenetic analysis, and motif identification. Transgenic maize lines were generated via Agrobacterium-mediated transformation and subjected to physiological, biochemical, and transcriptomic analyses under alkaline stress. Under alkaline stress, the results revealed that LcTprxII overexpression significantly preserved chlorophyll content, mitigated oxidative damage, and maintained growth compared to wild-type plants, as evidenced by elevated activities of antioxidant enz

SUBMITTER: Michael DPJ 

PROVIDER: S-EPMC12114990 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature