Evaluating the Immunogenic Potential of ApxI and ApxII from <i>Actinobacillus pleuropneumoniae</i>: An Immunoinformatics-Driven Study on mRNA Candidates.

ABSTRACT: Porcine infectious pleuropneumonia (PCP) caused by Actinobacillus pleuropneumoniae (APP) leads to severe economic losses in swine production. Commercial vaccines offer limited cross-protection for the 19 serotypes, while APP mRNA vaccines remain unexplored. This study evaluated eight candidate APP proteins (ApxI-IV, OlmA, TbpB, GalT, and GalU) using immunobioinformatics tools, and their immunogenicity and cross-protection were assessed in a mouse model. The results revealed that ApxI and ApxII excel due to their stability, strong antigenicity, non-sensitization, and high immune receptor affinity. Compared to the PBS group, both ApxI and ApxII induced higher serum IgG, IL-2, IL-4, and IFN-γ levels. Following challenge with the two most prevalent APP strains in Mainland China, APP 5b

SUBMITTER: Deng Y 

PROVIDER: S-EPMC12115502 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature